Long Que1, xiangchen che1, Nuhn Jacob1, Schneider Ian2
14:00 - 14:30 | Wed 26 Jul | Grand Ballroom #3 | WeO1O3.1
Herein a nanostructure-enabled fluorescence chip to monitor the cell secretion is reported for the first time. The fluorescence chip is based on nanostructured aluminum oxide or nanoporous anodic aluminum oxide (Nano-AAO), which can dramatically enhance the fluorescence signal compared to coverslip glass. As a proof of concept, transforming growth factor β1 (TGF-β1) secreted directly by pancreaticstellate (iTAF) cells in isolation and in co-culture with pancreatic cancer (capan-1) cells has been monitored by the sensors using a sandwich assay. It has been found that 10 ng/ml of TGFβ1 can be readily detected in the complexity of conditioned cell media. The successful detection of the TGFβ1 secreted by iTAF cells in conditioned media indicates the possibility of the fluorescence sensors to monitor the cell secretion in real time.