Conjugated Barcoded Particles for Multiplexed Biomarker Quantification with a Microfluidic Biochip

Abstract

Multiplexing is a method of analyzing multiple analytes in a biological assay in one single step. Multiplexing provides advantages of low sample processing time, small sample volume and low cost. One of the effective ways of doing multiplexing is by using the barcoded particles, which are fabricated by Stop Flow Lithography process in a microfluidic environment. The barcoded particles have the ability to generate distinct electrical signatures which makes it possible to detect multiple biomolecules in an assay. The barcoded particle can also be extended to detect multiple diagnostics and prognostic biomarkers in diseases like Sepsis, Acute Kidney Injury, and AIDS diagnostic and management.