Development of a Novel Isothermal Amplification Assay for Sensitive Detection of Plasmodium Falciparum Malaria

Abstract

Malaria infection continues to impact global health, and several countries are working toward eliminating the disease. Identification and treatment of asymptomatic infections is crucial for elimination, but currently available diagnostics are not sensitive enough to detect low density infections. Here, we have developed a novel isothermal DNA amplification technique with a simple visual readout to sensitively detect malaria infection with low parasite density (