Development of a Flow-Free Automated Microfluidic ELISA for Detecting ZIKA Virus NS1 Antigen at Point of Care

Md Alamgir Kabir1, Hussein Zilouchian, Waseem Asghar2

  • 1Florida Atlantic University (FAU)
  • 2Florida Atlantic University, Boca Raton

Details

12:30 - 14:30 | Thu 21 Nov | Upper Foyer Balcony | B1P-D.3

Session: Poster Session - Infectious Disease Diagnostics and Anti Microbial Resistance 2

Abstract

We have developed an automated magnetic actuation platform suitable for POC micro-a-fluidic Sandwich enzyme-linked immunosorbent assay using antibody-coated superparamagnetic beads. The immunoassay is developed for colorimetric detection of ZIKV NS1 antigen, which is spiked in whole plasma with a total assay time of ~10 minutes in a micro-a-fluidic chip. Our developed platform is easily deployable, high-throughput, low cost, and easily disposable which and can be used in resource-limited settings.