Point-of-Care Magnetofluidic Platform for Neisseria Gonorrhoeae Detection and Antimicrobial Resistance Determination

Jeff Wang1, Alexander Trick1

  • 1Johns Hopkins University

Details

12:30 - 14:30 | Thu 21 November | Room E1&2 | B1P-A.8

Session: [B1P-A] Technology Demonstrations

Category: Poster Session
Theme: Technology Demonstration Showcase

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Abstract

We have developed a portable magnetofluidic platform for diagnosis of Neisseria gonorrhoeae (NG) with simultaneous evaluation of antimicrobial susceptibility. Our instrument automates sample processing and nucleic acid amplification assays within a single disposable thermoplastic cartridge using transport of functionalized magnetic particles. The cartridges contain preloaded reagents for a duplexed PCR assay targeting an NG-specific opa gene and the wildtype gyrA gene indicating pathogen susceptibility to ciprofloxacin. Sample-to-answer results from our cartridge assays in 15 minutes enables potential for guiding clinician decisions at the point-of-care and promote antimicrobial stewardship with more diverse options for treatment.