A Nanosurface Microfluidic Device for Capture and Detection of Bacteria

Abstract

Here we report on design, fabrication and implementation of a nanosurface microfluidic device for bacteria capture. The device features a pillar-assisted self-assembly microparticles nano-filter and nano/micro gold islands for efficient capture and optical detection of bacteria, with potential point of care applications. The design was optimized using a 2D COMSOL simulation. We used a single lithography step for pattern generation. Next, we electrodeposited the nano/micro gold islands. Finally, we sealed the device using a polydimethylsiloxane (PDMS) and punched interfacing ports. We experimentally evaluated the device capture performance using Escherichia coli (E.coli) bacteria. The device showed efficient bacteria capture performance.