Neuronal Excitability and Network Formation on Optically Transparent Electrode Materials

Cort Thompson1, Sahar Khan1, Wasif Khan1, Wen Li1, Erin Purcell1

  • 1Michigan State University

Details

16:00 - 17:45 | Fri 26 May | Emerald III, Rose, Narcissus & Jasmine | FrPS2T1.2

Session: Poster II

Abstract

With the advent of genetically-encoded optical tools to trigger or report neuronal activity, new designs for multielectrode arrays (MEAs) used in neural interfacing incorporate both optical and electrical modes of stimulating or recording neural activity. Likewise, the need to improve upon the biocompatibility of implanted MEAs has moved the field towards the use of softer, more compliant materials in device fabrication. However, there is limited available information on the impact of the materials used in MEAs on the function of interfaced individual neurons and neuronal networks. We assessed the responses of rat cortical neurons on optically transparent materials commonly used in the construction of “next-generation” devices: indium tin oxide (ITO), parylene-C, and polydimethylsiloxane (PDMS). We found that neuronal network formation and spiking responses to electrical stimulation were enhanced in neurons cultured on ITO. We observed reduced excitability and synaptic connectivity between neurons cultured on PDMS. We hypothesize that the superior conductivity of ITO and suboptimal neuronal attachment to PDMS contributed to our results.