A Specific Multiplexed Loop-Mediated Amplification (mLAMP) Assay for Human Papillomavirus (HPV) 16 and 18

Winnie Wong1, Catherine M. Klapperich2

  • 1Boston University BME
  • 2Boston University

Details

12:00 - 14:00 | Thu 10 Nov | Maya Ballroom Foyer | ThPO.34

Session: HI-POCT Poster Session and POC Technologies Demonstrations

Abstract

Cervical cancer is a major health problem worldwide, especially in the developing world where standard screening techniques are not accessible. Cervical cancer is caused by HPV, with 70% from HPV 16 and 18[1]. Current HPV DNA tests are expensive and require complex instrumentation and highly trained personnel, preventing their use in low-resource settings. This paper reports a novel multiplexed loop-mediated amplification (mLAMP) assay to detect the DNA of human papillomavirus (HPV) 16 and 18. Differential probes are incorporated into the two strains and bind to specific lines on a paper lateral flow strip (LFS). Further, we confirm via sequencing that the amplified target E7 gene for human papillomavirus (HPV) 16 and 18 is specific. The assay uses a fluorescent gel imager and can be easily integrated into the standard LAMP workflow.